The antagonistic activity of the synbiotic containing Lactobacillus acidophilus and pineapple residue FOS against pathogenic bacteria / A atividade antagônica do simbiótico contendo Lactobacillus acidophilus e resíduo de abacaxi FOS contra bactérias patogênicas

Fructooligosaccharide is used widely in many foods and pharmaceutical industries and produced by using different ways such as extracting it from plants or producing it by using plants and microorganisms' enzymes. In a previous study, we extracted Fructosyltransferase (Ftase) enzyme from pineapple residue and produced FOS. In this study, we measured the antagonistic activity of two synbiotics, the first synbiotic containing Lactobacillus acidophilus and the produced FOS, the second synbiotic containing Lactobacillus acidophilus and standard FOS, against pathogenic bacteria (P. aeruginosa, E. coli, S. aureus and B cereus). The results showed that the antagonistic activity of both synbiotic types was very close, as there were no significant differences between them except in the antagonistic activity against S. aureus, there was a significant difference between the synbiotic containing the standard FOS, which was the highest in its antagonistic activity compared to the synbiotic containing the produced FOS in this study. The activity of the fructooligosaccharide (FOS) extracted from pineapple residue was evident in enhancing the activity of the probiotic bacteria (L. acidophilus), which had a major role in the production of acids and compounds that inhibited the pathogenic bacteria. The diameters of inhibition areas in the current study ranged between 19.33-28 mm, and E. coli was more susceptible to inhibition, followed by S. aureus, P. aeruginosa, and B. cereus, respectively.

O fruto-oligossacarídeo (FOS) é amplamente utilizado em muitos alimentos e indústrias farmacêuticas, e é produzido por meio de diferentes maneiras, como extraí-lo de plantas ou produzi-lo usando enzimas de plantas e microrganismos. Em um estudo anterior, extraímos a enzima frutosiltransferase (Ftase) do resíduo de abacaxi e produzimos FOS. Neste estudo, medimos a atividade antagônica de dois simbióticos: o primeiro simbiótico contendo Lactobacillus acidophilus e o FOS produzido, e o segundo simbiótico contendo Lactobacillus acidophilus e o FOS padrão, contra bactérias patogênicas (P. aeruginosa, E. coli, S. aureus e B. cereus). Os resultados mostraram que a atividade antagônica de ambos os tipos simbióticos foi muito próxima, pois não houve diferenças significativas entre eles, exceto na atividade antagônica contra S. aureus, em que houve uma diferença significativa entre o simbiótico contendo o FOS padrão, que foi o mais alto em sua atividade antagônica, em comparação com o simbiótico contendo o FOS produzido neste estudo. A atividade do fruto-oligossacarídeo (FOS) extraído do resíduo de abacaxi ficou evidente no aumento da atividade da bactéria probiótica (L. acidophilus), que teve papel importante na produção de ácidos e compostos inibidores das bactérias patogênicas. Os diâmetros das áreas de inibição no estudo atual variaram entre 19,33 e 28 mm, e E. coli foi mais suscetível à inibição, seguida por S. aureus, P. aeruginosa e B. cereus, respectivamente.

The antagonistic activity of the synbiotic containing Lactobacillus acidophilus and pineapple residue FOS against pathogenic bacteria

Abstract Fructooligosaccharide is used widely in many foods and pharmaceutical industries and produced by using different ways such as extracting it from plants or producing it by using plants and microorganisms enzymes. In a previous study, we extracted Fructosyltransferase (Ftase) enzyme from pineapple residue and produced FOS. In this study, we measured the antagonistic activity of two synbiotics, the first synbiotic containing Lactobacillus acidophilus and the produced FOS, the second synbiotic containing Lactobacillus acidophilus and standard FOS, against pathogenic bacteria (P. aeruginosa, E. coli, S. aureus and B cereus). The results showed that the antagonistic activity of both synbiotic types was very close, as there were no significant differences between them except in the antagonistic activity against S. aureus, there was a significant difference between the synbiotic containing the standard FOS, which was the highest in its antagonistic activity compared to the synbiotic containing the produced FOS in this study. The activity of the fructooligosaccharide (FOS) extracted from pineapple residue was evident in enhancing the activity of the probiotic bacteria (L. acidophilus), which had a major role in the production of acids and compounds that inhibited the pathogenic bacteria. The diameters of inhibition areas in the current study ranged between 19.33-28 mm, and E. coli was more susceptible to inhibition, followed by S. aureus, P. aeruginosa, and B. cereus, respectively.

Resumo O fruto-oligossacarídeo (FOS) é amplamente utilizado em muitos alimentos e indústrias farmacêuticas, e é produzido por meio de diferentes maneiras, como extraí-lo de plantas ou produzi-lo usando enzimas de plantas e microrganismos. Em um estudo anterior, extraímos a enzima frutosiltransferase (Ftase) do resíduo de abacaxi e produzimos FOS. Neste estudo, medimos a atividade antagônica de dois simbióticos: o primeiro simbiótico contendo Lactobacillus acidophilus e o FOS produzido, e o segundo simbiótico contendo Lactobacillus acidophilus e o FOS padrão, contra bactérias patogênicas (P. aeruginosa, E. coli, S. aureus e B. cereus). Os resultados mostraram que a atividade antagônica de ambos os tipos simbióticos foi muito próxima, pois não houve diferenças significativas entre eles, exceto na atividade antagônica contra S. aureus, em que houve uma diferença significativa entre o simbiótico contendo o FOS padrão, que foi o mais alto em sua atividade antagônica, em comparação com o simbiótico contendo o FOS produzido neste estudo. A atividade do fruto-oligossacarídeo (FOS) extraído do resíduo de abacaxi ficou evidente no aumento da atividade da bactéria probiótica (L. acidophilus), que teve papel importante na produção de ácidos e compostos inibidores das bactérias patogênicas. Os diâmetros das áreas de inibição no estudo atual variaram entre 19,33 e 28 mm, e E. coli foi mais suscetível à inibição, seguida por S. aureus, P. aeruginosa e B. cereus, respectivamente.

Enzyme-linked Immunosorbent Assay in Quality Control of Chinese Medicines： A Review / 中国实验方剂学杂志

In the quality control of Chinese medicine， the detection of active components and toxic and harmful components are two important links. Although conventional methods such as high performance liquid chromatography and liquid chromatography-mass spectrometry can accurately quantify the above substances， they have shortcomings such as complicated operation， high costs， inability of detection at any time， difficult detection of insoluble and macromolecular substances. Enzyme-linked immunosorbent assay （ELISA） can adsorb antigens or antibodies on the surface of solid carriers and realize qualitative or quantitative analysis of targets by using the specific reactions of antigens and antibodies. This method is praised for the simple operation， high sensitivity， strong specificity， simple requirements for experimental equipment， a wide application range， and low costs. In recent years， ELISA has been widely used in the quality control of Chinese medicine， especially in the content determination of mycotoxins represented by aflatoxin and the qualitative and quantitative analysis of active components. ELISA plays an increasingly important role with its unique advantages， providing new methods and ideas for the rapid quality examination of large quantities of Chinese medicines. This paper reviews the research progress in ELISA for the quality control of Chinese medicine in recent years and prospects its technical development and application prospects， aiming to provide reference and research ideas for further using this method to ensure the quality， safety， and controllability of Chinese medicine.

Determination of antibiotic residues in chicken meat using ultra-high-performance liquid chromatography coupled with hybrid quadrupole-orbitrap high-resolution mass spectrometry / 预防医学

Objective@#To optimize the sample pretreatment and establish an ultra-high-performance liquid chromatography coupled with hybrid quadrupole-orbitrap high-resolution mass spectrometry (UPLC-Q-Orbitrap-MS) assay based on the parallel reaction monitoring (PRM) mode for determination of antibiotic residues in chicken meat.@*Methods@#Blank matrix-spiked chicken meat samples were extracted with 95% acetonitrile aqueous solution containing Na2EDTA and formic acid. The extraction solutions were cleaned up using different combinations of C18, PSA and GCB fillers, and the combinations with a higher antibiotic recovery rate was screened. The residues of 32 antibiotics were determined using UPLC-Q-Orbitrap-MS based on the PRM mode.@*Results@#If the extraction solution was cleaned up using the C18 filler, the largest number of antibiotics with a spiked recovery rate of >80% was seen, with matrix effects of 82.2% to 112.6%. The detection limits of 32 antibiotics were 0.8 to 5.8 μg/kg, with linear correlation coefficients of >0.99, spiked recovery rates of 71.3% to 111.5% and relative standard deviations of 3.2% to 14.2%.@*Conclusion@#The UPLC-Q-Orbitrap-MS assay is suitable for determination and quantitative analysis of multiple antibiotics in chicken meat. Key words： high-resolution mass spectrometry orbitrap antibiotic residue parallel reaction monitoring

Monitoring results of veterinary drug residues in chicken and eggs in Xinjiang in 2019 - 2020 / 公共卫生与预防医学

Objective To know the residues of 13 veterinary drug residues in chicken and eggs foods in some areas in Xinjiang. Methods A total of 170 chicken and egg samples were randomly selected from supermarkets and farmers' markets in seven cities in Xinjiang. Eleven quinolone antibiotics, two tetracycline antibiotics, ribavirin and metronidazole were examined for veterinary drug residues using liquid chromatography tandem mass spectrometry (LC-MS/MS) . Results The overall detection rate of veterinary drug residues in eggs and chicken were 20%（18/90）and11.25%（9/80）. The overall over-standard rate were 18.89%（17/90）and 0（0/80）. Veterinary drug residues in chicken are heavier than eggs. Veterinary drugs were detected and over-standarded in all seven cities in Xinjiang monitored. Conclusion The veterinary drug contamination in chicken and eggs in Xinjiang is relatively serious. It is recommended to strengthen the standardization of production and supervision to ensure food safety.

Analysis of the results of an international proficiency testing program for veterinary drug residue determination in food / 上海预防医学

ObjectiveTo evaluate the proficiency and consistency of domestic and foreign testing institutions in the field of veterinary drug residue detection in food， and to promote international cooperation and mutual recognition of testing results among these institutions. MethodsA robust statistical analysis was conducted on the testing results of 20 laboratories in eight countries and regions across North America， Europe， and Asia. The laboratories’ testing capabilities were evaluated using Z-score comparison. ResultsAmong the 20 participating laboratories， 18 achieved satisfactory results， resulting in a satisfaction rate of 90%， while 2 laboratories （10%） failed to meet the requirements. The satisfaction rate of domestic laboratories （100%） was higher than that of foreign laboratories （81.8%）. ConclusionDomestic laboratories perform better than overseas laboratories in determining veterinary drug residues in food. To enhance testing capabilities， these overseas laboratories with unsatisfactory evaluation results should strengthen their daily quality control and ensure traceability of original records.

Prokaryotic expression and activity detection of insulin-degrading enzyme mutant T142A / 中国生物制品学杂志

@#Objective To express insulin-degrading enzyme(IDE)mutant T142A in prokaryotic cells and detect its activity.Methods According to the results of multi-sequence alignment and IDE substrate co-crystal structure,an active residue in β6-strand structure of IDE were predicted.The recombinant plasmid ppSUMO-T142A,with the site mutation of threonine 142 to alanine,was constructed by point mutation technique and expressed by E.coli prokaryotic expression system.After purification by nickel ion column affinity chromatography,ion exchange chromatography and gel filtration chromatography,the mutant T142A was obtained and determined for the activity by fluorescence method.Results IDE amino acid sequence is highly conserved among 16 species.T142 directly participates in substrate binding,interacts with substrate cleavage sites,and is close to important structures such as catalytic active sites and door-subdomains.The mutation of recombinant plasmid ppSUMO-T142A was proved to be correct by sequencing.The expressed fusion protein His-SUMO-T142A was mainly existed in soluble form in the supernatant at a concentration of 18 mg/mL,with a relative molecular mass of about 131 000;After three steps of purification,the purity of mutant T142A reached 86%.The maximum reaction rate(V_(max))of T142A catalytic degradation of fluorescent substrate V was 501.06 min~(-1) and the Michaelis constant(K_m) was 9.01μmol/L.Compared with wild-type IDE(V_(max) was 2 814.32 min~(-1),K_m was 11.93μmol/L),the activity of T142A decreased significantly.Conclusion The activity of IDE mutant T142A expressed in this study greatly decreases,while T142 is an important residue for IDE to play its enzymatic function,which provides an experimental basis for the development of new IDE activity regulatory molecules.

Development and verification of high performance liquidchromatography for determination of ethylenediaminetetraacetic acid disodium salt residue in NMM tumor DNA vaccine / 中国生物制品学杂志

@#Objective To develop and verify a high performance liquid chromatography(HPLC) method for the determination of ethylenediaminetetraacetic acid disodium salt(EDTA-2Na) residues in the bulk of NMM tumor DNA vaccine for the quality control of DNA vaccine.Methods After NMM tumor DNA vaccine bulk was complexed with copper sulfate,a HPLC method for the determination of EDTA-2Na residues was developed with Agilent ZORBA XSB-C18(150 mm × 4.6 mm,5 μm) as the chromatographic column,water,tetrabutylammonium hydroxide 10% and acetonitrile solution(74.5:0.5:25)as the mobile phase.The detection method was as follows:the detection wavelength was 254 nm,the flow rate was 0.8 mL/min,the column temperature was 20 ℃ and the injection volume was 20 μL.The method was verified for the specificity,linear range,limit of detection(LOD),limit of quantification(LOQ),solution stability,durability,accuracy and precision,and used to detect the EDTA-2Na residues in several batches of DNA vaccine bulk.Results When EDTA-2Na control and DNA vaccine bulk with EDTA-2Na reacted with copper sulfate,the absorption peak appeared at around 5.3 min,while no absorption peak was observed when DNA vaccine bulk reacted with copper sulfate;In the range of 4～400 μg/mL,the control solution concentration showed a good linear relationship with the peak area,R~2=0.999 9;The LOD of the method was 10 ng/mL,and the LOQ was 40 ng/mL;The solution of control and sample was stable after placed for 12 h;When the detection conditions changed slightly(different mobile phase ratio,flow rate and column temperature),the influence on the detection results was within acceptable range;The average recovery rate of EDTA-2Na in low,medium and high concentration standard added samples was 101.38% with the RSD of 0.39%;0.1 mg/mL control solution was injected continuously for 6 times,and the peak area RSD was 0.04%.EDTA-2Na was not detected in 6 sample solution,and the peak area RSD of DNA vaccine bulk with EDTA-2Na solution was 0.02%,indicating a good intermediate precision.EDTA-2Na residue was not detected in these batches of DNA vaccine bulk.Conclusion The developed method is simple,accurate,reliable with good specificity,which can be used for the determi-nation of EDTA-2Na residues in DNA vaccine bulk.

Persistent Intraocular Residue with the Use of Dexycu® in Cataract Extraction: A Case Series

Purpose: The objective of this article is to report 8 cases of persistent IOL residue associated with the use of Dexycu® in the context of cataract surgery and then to subsequently describe each patients clinical course.Observations and Presentation: Between 2020-2021, persistent residue was noted in 8 eyes of 7 patients who received Dexycu® implants after cataract surgery. The residue was identified an average of 1.63 months after surgery (range 0.20-4.23). A subsequent procedure removed the residue from the intraocular lens; the average time to the follow-up procedure after surgery was 4.71 months (range 1.90-11.20).Conclusions and Importance: The Bausch and Lomb intraocular lenses seem to be predisposed to a Dexycu® persistent opacification, however correlation does not equate with causation. This article documents cases of persistent IOL residue with the use of Dexycu® and the MX60 lenses and its toric varieties. Further evaluation is necessary to elucidate the mechanism and risk factors for this occurrence.

Comparison of Pesticide Residue Levels in Tomatoes from Open Fields, Greenhouses, Markets and Consumers in Kirinyaga County, Kenya

The study was carried out to determine and compare pesticide residue levels in tomatoes from Mwea Irrigation Scheme. Thirty five tomato samples of Rambo variety randomly collected from open fields, greenhouses, markets and consumers were analyzed using QuEChERS method. Results showed that pesticide residue leve;s from greenhouse tomatoes was higher compared to open fields, markets and consumers. Alpha-cypermethrin level in greenhouse tomatoes (0.0871±0.0087mg/kg) was significantly (p<0.01) higher than from consumers (0.0218±0.0061mg/kg) while difenoconazole from greenhouse tomatoes (0.2597±0.0522 mg/kg) was significantly (p<0.05) higher than from the open field (0.0295±0.0014 mg/kg). Carbendazim level in greenhouse (1.2341±0.1667 mg/kg) tomatoes was significantly (p<0.001) higher than from open fields (0.0596±0.0178 mg/kg), markets (0.1160±0.0490 mg/kg) and consumers (0.0494±0.0155 mg/kg). Imidacloprid in greenhouse tomatoes (0.1446±0.0086 mg/kg) was significantly (p<0.001) higher than from the markets (0.0236±0.0019 mg/kg) and consumers (0.0170±0.0017 mg/kg). High pesticide residue levels in tomatoes are a health concern for consumers. Enforcing the food safety laws, enhancing farmer training on safe use of pesticides and creating awareness on pesticide risks would promote production of uncontaminated crops consumed locally.

Caracterização físico-química da farinha e dos extratos das sementes de abacate (Persea americana (Mill.) Lauraceae)

Introdução: as indústrias de processamento de frutas geram grande quantidade de biomassa residual que poderia ser reaproveitada. Considerando o elevado volume de resíduos produzidos pelo descarte das sementes do abacate e destacando o alto teor de compostos bioativos, é um produto interessante para ser avaliado. Objetivo: avaliar a composição centesimal da farinha das sementes de abacate e as propriedades antioxidantes e antimicrobianos dos seus extratos. Métodos: os extratos foram obtidos por maceração da farinha do caroço do abacate desidratada em diferentes temperaturas (4,25 e 60 °C) utilizando n-hexano e etanol como solventes. Resultados: verificou-se que a farinha é uma excelente fonte de carboidratos, com alto teor de fibras, proteínas e minerais (N, K, Mg e Ca, entre outros). A temperatura da extração influenciou tanto no rendimento como no conteúdo de fenóis totais, atividades antioxidantes e antimicrobianas dos extratos. Os extratos etanoicos obtidos à 60 °C apresentaram maior rendimento (18%) e teor de compostos fenólicos totais (~840 mgEAG/g). Também os extratos etanoicos apresentaram maior atividade antioxidante (IC50= 0,013 mg/mL e 0,018 mg/mL) em temperaturas mais baixas de extração, 4 °C e 25 °C, respectivamente. Já extrato hexanóico obtido à 4 °C apresentou maior atividade antimicrobiana para as quatro bactérias testadas (L. monocytogenes, S. aureus, S. choleraesuis e E. coli). Conclusão: a farinha obtida das sementes de abacate, apresentam alto valor biológico e podem ser usadas como suplementos na alimentação humana.

SUMMARY Introduction: The fruit processing industries generate a large amount of residual biomass that could be reused. Considering the high volume of residues produced by the disposal of avocado seeds and highlighting the high content of bioactive compounds. Aim: To evaluate the centesimal composition of avocado seed flour and the antioxidant and antimicrobial properties of its extracts. Methodology: The extracts were obtained by macerating the avocado seed flour dehydrated at different temperatures (4,25 and 60 °C) using n-hexane and ethanol as solvents. Results: The flour is an excellent source of carbohydrates, with a high content of fibers, proteins and minerals (N, K, Mg and Ca, among others). The extraction temperature has influence in the yield and the content of total phenols, antioxidant and antimicrobial activities of the extracts. The ethanolic extracts obtained at 60 °C showed high yield (18%) and content of total phenolic compounds (~840 mg GAE/g). In addition, ethanol extracts showed high antioxidant activity (IC50= 0.013 mg/mL and 0.018 mg/mL) at lower extraction temperatures, 4 °C and 25 °C, respectively. Hexanoic extract obtained at 4 °C, on the other hand, showed greater antimicrobial activity for the four tested bacteria (L. monocytogenes, S. aureus, S. choleraesuis and E. coli). Conclusion: The flour obtained from avocado seeds has a high biological value and can be used as supplements in human food.

Introducción: las industrias de procesamiento de frutas generan una gran cantidad de biomasa residual que podría reutilizarse. Considerando el alto volumen de residuos que produce la disposición de semillas de aguacate y destacando el alto contenido de compuestos bioactivos. Objetivo: evaluar la composición centesimal de la harina de semilla de aguacate y las propiedades antioxidantes y antimicrobianas de sus extractos. Metodología: los extractos se obtuvieron macerando la harina de semilla de aguacate deshidratada a diferentes temperaturas (4, 25 y 60 °C) utilizando n-hexano y etanol como solventes. Resultados: la harina es una excelente fuente de carbohidratos, con un alto contenido en fibras, proteínas y minerales (N, K, Mg y Ca, entre otros). La temperatura de extracción influye en el rendimiento y el contenido de fenoles totales, actividades antioxidantes y antimicrobianas de los extractos. Los extractos etanólicos obtenidos a 60 °C mostraron alto rendimiento (18%) y contenido de compuestos fenólicos totales (~ 840 mg GAE/g). Además, los extractos de etanol mostraron una alta actividad antioxidante (IC50 = 0,013 mg/mL y 0,018 mg/mL) a temperaturas de extracción más bajas, 4 °C y 25 °C, respectivamente. El extracto hexano obtenido a 4 °C, en cambio, mostró mayor actividad antimicrobiana para las cuatro bacterias ensayadas (L. monocytogenes, S. aureus, S. choleraesuis y E. coli). Conclusión: la harina obtenida a partir de semillas de aguacate tiene un alto valor biológico y puede ser utilizada como complemento en la alimentación humana.

A Case Report to Study The Effective Role of Varunadi Kashaya and Dashmool Churna in the Causes of Increased Post Void Residual Urine

Post-Void Residual (PVR) volume of urine is the amount of urine retained in the bladder after a voluntary void that is measured by catheterization or non-invasively by ultrasonography. The increased value of PVR act as a diagnostic tool in a urological pathology such as a neurological disease/injury to the bladder, mechanical obstruction, infection or medication induced urinary retention. A PVR less than 50ml signifies adequate bladder emptying. The present case is of 3.1mm non-obstructing left renal calculus and Grade III prostatomegaly with increased Post Void Residual urine (91ml). Renal calculi and prostatomegaly, both are common obstructive uropathies which interferes with the normal outflow of urine. Treatment is aimed at relieving the symptoms and to treat the underlying cause as well. The treatment strategies that can be followed are catheterization, stenting, surgery, lithotripsy, hormonal therapy and antibiotic therapy. The Ayurvedic formulation, Varunadi Kashaya and Dashmool Churna are Vata kapha pacifying drugs. With its Mutrala, Deepana, Anulomana, Shothghana, Shoolghana properties, it had successfully eliminated renal calculus, decreased the prostate size and ultimately, lowered the PVR value as evidenced in USG report. There is a considerable relief in the troublesome urological symptoms- dysuria, dribbling micturition, weak urine stream, inadequate bladder emptying and abdominal pain.

Contribution of the carbonized rice husk added to the substrate in the production of vegetable seedlings

The carbonized rice husk (CRH) is an agro-industrial residue with great potential for use in mixtures with other substrates for the production of vegetable seedlings. Thus, the purpose of this study was to evaluate the effect of the addition of CRH to the commercial substrate for the production of greenhouse seedlings. The evaluated vegetables were lettuce, broccoli and mustard, cultivated in polystyrene foam (Styrofoam®) trays. Five treatments were evaluated: 100% of the commercial substrate (CS); 25% of CRH + 75% of CS; 50% of CRH + 50% of CS; 75% CRH + 25% CS; and 100% CRH, in a completely randomized design with twelve replicates. The substrates were evaluated for their physical and chemical characteristics. The morphometric evaluations were: number of leaves per plant, plant height, shoot fresh matter yield, root length, and shoot and root dry matter yield. The data were submitted to regression analysis, and the homogeneity of the variance between the experiments was confirmed by the Cochran test. With the addition of CRH to the substrate, there was an increase in total porosity and P content and a reduction in density, water holding capacity, cation exchange capacity, electrical conductivity, and N, K, S, Ca and Mg contents. The carbonized rice husk added to the commercial substrate is a viable alternative for the production of lettuce, broccoli and mustard seedlings. The quality of the vegetable seedlings was higher when there was a 25% CRH + 75% CS mixture.

Research progress on detection methods of pyrethroid insecticide residues in human biomaterials / 环境与职业医学

In recent years, due to the wide use of pyrethroid insecticides, the impact of pyrethroid insecticides on human health cannot be ignored. Therefore, how to detect pyrethroid insecticide residues in human body accurately and efficiently has become a research hotspot. However, it is difficult to detect pyrethroid insecticides because of its low residues and fast metabolism. Our article reviewed research on the detection of pyrethroid pesticide residues in human biomaterials in recent years, listed various pretreatment methods and detection methods of different biomaterials in detail, as well as summarized reported limits of detection, limits of quantitation, and recoveries of different methods. Finally, we prospected further development in detection methods of pyrethroid pesticide residues in human biomaterials.

Associação entre o número de deglutições, resíduo faríngeo e broncoaspiração na esclerose múltipla / Association between the number of swallowing, pharyngeal residue and bronchopulmonary aspiration in multiple sclerosis

RESUMO Objetivo verificar a associação entre o número de deglutições e presença de resíduo faríngeo e broncoaspiração em pessoas com esclerose múltipla. Métodos estudo transversal observacional de exames de videofluoroscopia de 231 deglutições de indivíduos com esclerose múltipla. Três fonoaudiólogas avaliaram as deglutições de IDDSI 1 (International Dysphagia Diet Standardisation Initiative) (5 ml e 10 ml) e IDDSI 4 (8 ml) quanto à presença de resíduo faríngeo e de penetração/aspiração. Deglutições que não apresentaram resíduo faríngeo foram classificadas como deglutições sem resíduos faríngeos (DSR) e as que apresentaram, como deglutições com resíduos faríngeos (DCR), sendo estas últimas subdivididas em resíduos faríngeos em todas as ofertas ou eventuais (DCR1 e DCR2). O número de deglutições foi analisado por um avaliador cego e comparado com os dados demográficos e clínicos. Resultados das 231 deglutições, 73 (31,6%) apresentaram resíduos faríngeos. O número médio de deglutições foi semelhante nas deglutições sem e com resíduos faríngeos em cada consistência e volume e nas variáveis idade, gênero, tipo de esclerose múltipla e incapacidade funcional. Houve associação entre a média do número de deglutições e a ausência de penetração/aspiração, quando comparada às deglutições sem e com resíduos faríngeos, nas DCR2 e em indivíduos acima de 50 anos. Ao analisar intragrupo, observou-se associação nas DCR, sendo maior na ausência de penetração/aspiração e nas DCR2. Conclusão não houve correlação entre o número de deglutições e a presença de resíduos em recessos faríngeos na esclerose múltipla. Todavia, o número de deglutições foi maior quando houve resíduo e ausência de disfagia e de penetração/aspiração, em indivíduos mais velhos.

ABSTRACT Purpose To verify the association between the number of swallows and the presence of pharyngeal residue and bronchoaspiration in people with Multiple Sclerosis. Methods An observational cross-sectional study of videofluoroscopic examinations of 231 swallows from individuals with Multiple Sclerosis. Three speech therapists evaluated IDDSI 1 (International Dysphagia Diet Standardisation Initiative) (5ml and 10ml) and IDDSI 4 (8ml) deglutitions for pharyngeal residue and penetration/ aspiration. Swallows with no pharyngeal residue were classified as swallows without pharyngeal residue (SWTR) and those with pharyngeal residue (SWR), the latter subdivided into pharyngeal residue in all or occasional offerings (SWR1 e SWR2). The number of swallows was analyzed by a blind evaluator and compared with demographic and clinical data. Results Of the 231 swallows, 73 (31.6%) showed pharyngeal residues. The mean number of swallows was similar in the deglutitions with and without pharyngeal residues in each consistency and volume and in the variables age, gender, type of Multiple Sclerosis and functional disability. There was an association between the mean number of swallows and the absence of penetration/aspiration when comparing deglutitions with and without pharyngeal residues, in SWR2 and in individuals over 50 years of age. When analyzing intragroup, an association was observed in SWR, being higher in the absence of penetration/aspiration and in SWR2. Conclusion There was no correlation between the number of swallows and the presence of residues in pharyngeal recesses in multiple sclerosis. However, the number of swallows was higher when there was residue and absence of dysphagia and penetration/aspiration, and in older individuals.

Effect of Daoqi Tongjing needling method on intra-uterine residue after drug abortion from the perspectives of Hegu (LI 4) and Sanyinjiao (SP 6) / 中国针灸

The paper introduces professor ZHUANG Li-xing's clinical experience in treatment of intra-uterine residue and lochia after drug abortion. Professor ZHUANG believes that this disorder is related to "dysfunction of the thoroughfare vessel and the conception vessel, qi deficiency and blood stagnation, and retention of turbid qi in the uterus" in pathogenesis. The treating principle should focus on "regulating the functions of the thoroughfare vessel and the conception vessel, tonifying qi and eliminating stasis, as well as promoting qi movement". Besides Hegu (LI 4) and Sanyinjiao (SP 6), the acupoints are added from the conception vessel. The Daoqi Tongjing needling technique (the specific technique for directing qi and preserving essence) is exerted flexibly instead of traditional reinforcing and reducing technique of acupuncture to tonify qi and remove stasis.

High-throughput screening of drug residues in aquatic products by liquid chromatography-electrostatic field track well high resolution mass spectrometry / 上海预防医学

ObjectiveIn order to ensure the food safety of cultured aquatic products in China， strengthen the supervision and improve detection efficiency， a high-throughput screening method for drug residues in aquatic products by mass spectrometry is to be developed. MethodsLiquid chromatography-electrostatic field track well high resolution mass spectrometry and C18 column were used to separate and collect the information of ion fragments and retention time of 195 veterinary drug standards in positive ion mode， and drug residues in aquatic products were extracted by organic solvents. ResultsThe mass spectrum database and detection methods of 195 veterinary drug standards were established， and a pre-treatment method was developed for extracting veterinary drug residues from aquatic products by acetonitrile and ethyl acetate. The detection method was sensitive， accurate， simple and rapid， and the adding standard recovery of more than 80% compounds reached 70%‒110%. ConclusionThe establishment of this method can quickly and massively screen drug residues in aquatic products， provide technical support for food safety risk monitoring and supervision， cope with the increasing demand for aquatic products and aquaculture volume year by year， and provide a theoretical basis for the subsequent development of mass spectrometry high-throughput detection methods.

Validation of detection method for tributyl phosphate residue in human prothrombin complex / 中国输血杂志

【Objective】 To establish gas chromatography for the determination of tributyl phosphate(TBP) residues in human prothrombin complex and then verify it. 【Methods】 Acid modified polyethylene glycol(PEG)(20M) capillary column was used with n-hexane as solvent. The chromatographic parameters were as follows: gasification chamber temperature at 220 ℃, column temperature at 155 ℃, detector temperature at 220 ℃, column flow rate at 2.0 mL/min, carrier gas as N2, detector as FID, and collection time for 10min. The accuracy, repeatability, linearity, specificity, intermediate precision, detection limit, quantitative limit, range and durability were verified. 【Results】 The verification results showed that the method had good specificity. The linear regression correlation coefficient of standard curve was 0.999 90. The recovery rate were 98.4%, 97.5% and 95.7% when the concentration at 50%, 100%(30μg/mL) and 150%, respectively, with an average recovery of 97.2% and a relative deviation of 2.15%. When the concentration was 100%, the repeatability was 2.08%, and the relative deviation of intermediate precision was 1.63%. The detection limit was 0.255 μg/mL, and the quantitative limit was 0.511 μg/mL. After changing capillary chromatographic columns with different batch numbers but the same types and manufacturer, the applicability test of the system met the requirements, and the method had good durability. 【Conclusion】 This method can be used for the determination of TBP residues of human prothrombin complex in laboratory.

Estudo comparativo dos bio-óleos obtidos por pirólises rápida e lenta do caroço de pêssego / Comparative study of bio-oils obtained by fast and slow pyrolysis of peach stone

RESUMO O processamento de pêssego em conserva gera o caroço como resíduo agroindustrial de difícil destinação. Assim, a técnica de pirólise do endocarpo lenhoso é um eficiente tratamento para conversão da biomassa em produtos com valor agregado. Entre os produtos, o bio-óleo é composto de duas fases imiscíveis (orgânica e aquosa), com potencial para suprir a produção de produtos químicos em diferentes ramos industriais. O objetivo deste estudo foi obter e avaliar a constituição da fase orgânica dos bio-óleos com base nas pirólises rápida (reator de quartzo) e lenta (reator de aço inox) do endocarpo lenhoso, ambos de leito fixo a 700°C evidenciando assim a influência dos processos na constituição dos bio-óleos obtidos. As frações líquidas foram derivatizadas com N-metil-N-trimetilsililtrifluoracetamida para posterior caracterização em cromatografia gasosa acoplada à espectrometria de massas. Pela metodologia empregada, detectaram-se nas fases orgânicas dos bio-óleos da pirólise rápida e lenta um total de 59 e 41 compostos, respectivamente; destes, os fenóis são majoritários com 59,9% e 67,2%, em massa, em que os núcleos metoxifenol (rápida) e os alquilfenóis (lenta) são os compostos predominantes. Conclui-se que a etapa de derivatização e a técnica de cromatografia gasosa acoplada à espectrometria de massas foram satisfatórias para a caracterização qualitativa e semiquantitativa dos bio-óleos, os quais mostraram potencial de utilização como fonte de produtos químicos e beneficiamento no reaproveitamento de resíduos.

ABSTRACT The processing of pickled peaches generates the peach stone as an agro-industrial residue that is difficult to dispose of. Thus, the woody endocarp pyrolysis technique is an efficient treatment for converting biomass into value-added products. Among the products, bio-oil is composed of two immiscible phases (organic and aqueous), with potential to supply the production of chemical products in different industrial sectors. The objective of this study was to obtain and evaluate the constitution of the organic phase of the bio-oils based on the fast (quartz reactor) and slow (stainless steel reactor) pyrolysis of the woody endocarp, both with fixed bed at 700°C, thus evidencing the influence of processes in the constitution of the obtained bio-oils. As liquid fractions, they were derived with N-methyl-N-trimethylsilyltrifluoracetamide for further characterization in gas chromatography coupled with mass spectrometry. Through the methodology employed, a total of 59 and 41 compounds were detected in the organic phases of the fast and slow pyrolysis bio-oils, respectively; Phenols are the majority, with 59.9 and 67.2% by weight, where the methoxyphenol (fast) and cresols (slow) are the predominant compounds. It is concluded that the derivatization stage and the gas chromatography/mass spectrometry technique were satisfactory for the qualitative and semi-quantitative characterization of bio-oils, which they can potentially use as a source of chemical products and to benefit from reuse of residue.

Pequi (Caryocar brasiliense Camb.) by-product for lambs: Performance, feed intake, and nutrient digestibility / Subproducto de pequi (Caryocar brasiliense Camb.) para corderos: Desempeño, consumo y digestibilidad de nutrientes / Subproduto de pequi (Caryocar brasiliense Camb.) em cordeiros: Desempenho, consumo e digestibilidade dos nutrientes

Abstract Background: The use of pequi by-product as animal feed is an appealing alternative to reduce overall production costs. However, little is known regarding its effects on animal performance as well as the ideal inclusion level in the diet. Objective: To evaluate performance, intake, and digestibility of confined lambs receiving diets containing different levels of pequi by- product as a partial substitute for corn silage. Methods: The experiment was conducted in Curvelo, Brazil, using twenty-five crossbred Santa Inês male lambs (17.83 ± 1.73 kg) aged six months for 92 days. Increasing levels of pequi by-product (0, 6, 12, 18, and 24%) replacing corn silage were evaluated in a completely randomized design. Analysis of variance and regression were performed, with 5% significance level. Results: Inclusion of pequi by-product did not influence (p>0.05) average daily gain, total weight gain, or feed conversion ratio. No effect (p>0.05) was also observed on intake of dry matter, organic matter, ether extract, crude protein, neutral detergent fiber, total carbohydrates, and total digestible nutrients. However, a linear decrease was observed (p<0.05) in the apparent digestibility of dry matter, organic matter, ether extract, crude protein, neutral detergent fiber, total carbohydrates, and total digestible nutrients with increasing inclusion of pequi by-product. Conclusion: Pequi by-product is a potential substitute for corn silage in lamb diets because it does not influence animal performance and feed intake. However, digestibility decreases with increasing dietary inclusion levels of pequi by-product.

Resumen Antecedentes: la inclusion de subproductos de pequi en alimentación animal es una estrategia atractiva para reducir los costos de producción. Sin embargo, todavía se sabe poco sobre el efecto de este subproducto en el rendimiento de los animales, así como su nivel ideal de inclusión en la dieta. Objetivo: evaluar el rendimiento, consumo y digestibilidad en corderos confinados recibiendo dietas con subproducto de pequi en reemplazo parcial del ensilado de maíz. Métodos: el experimento se realizó en Curvelo, Brasil, utilizando 25 corderos machos cruzados de raza Santa Inês (17,83 ± 1,73 kg) y 6 meses de edad, durante 92 días. Se evaluaron niveles crecientes del subproducto de pequi (0, 6, 12, 18 y 24%) para sustituir el ensilado de maíz siguiendo un diseño completamente aleatorio. Los análisis de varianza y regresión se llevaron a cabo usando un nivel de significancia del 5%. Resultados: la inclusión del subproducto de pequi no influyó (p>0,05) en la ganancia de peso diaria, ganancia de peso total, ni conversión alimenticia. No se observó efecto (p>0,05) en la ingesta de materia seca, materia orgánica, extracto etéreo, proteína bruta, fibra detergente neutra, carbohidratos totales y nutrientes digestibles totales. Sin embargo, se observó una reducción lineal (p<0,05) en la digestibilidad aparente de materia seca, materia orgánica, extracto etéreo, proteína bruta, fibra detergente neutra, carbohidratos totales y nutrientes digestibles totales con el aumento del nivel de inclusión del subproducto. Conclusión: el subproducto de pequi puede utilizarse como sustituto parcial del ensilado de maíz, ya que no afecta la ganancia de peso ni la ingesta de nutrientes. Sin embargo, la digestibilidad disminuye al aumentar el nivel de inclusión del subproducto en la dieta.

Resumo Antecedentes: o uso de subprodutos do pequi na alimentação animal é uma estratégia atraente que busca a redução dos custos de produção. Entretanto, pouco ainda se sabe sobre o efeito desse subproduto no desempenho animal assim como qual seria o nível ideal da sua inclusão na dieta. Objetivo: avaliar o desempenho, consumo e digestibilidade em cordeiros confinados recebendo dietas contendo subproduto de pequi em substituição à silagem de milho. Métodos: o experimento foi conduzido em Curvelo, Brasil, utilizando 25 cordeiros machos cruzados da raça Santa Inês (17,83 ± 1,73 kg) com 6 meses de idade durante 92 dias. Níveis crescentes do subproduto de pequi (0, 6, 12, 18 e 24%) foram avaliados em substituição à silagem de milho em um delineamento inteiramente casualizado. A análise de variância e regressão foram conduzidas adotando 5% como nível de significância. Resultados: a inclusão do subproduto de pequi não influenciou (p>0,05) o ganho de peso médio diário, o ganho de peso total e a conversão alimentar. Também não foi observado efeito (p>0,05) no consumo de matéria seca, matéria orgânica, extrato etéreo, proteína bruta, fibra em detergente neutro, carboidratos totais e nutrientes digestíveis totais. Entretanto, foi observada redução linear (p<0,05) na digestibilidade aparente da matéria seca, matéria orgânica, extrato etéreo, proteína bruta, fibra em detergente neutro, carboidratos totais e nutrientes digestíveis totais com o aumento da inclusão do subproduto. Conclusão: o subproduto de pequi pode ser utilizado como substituto parcial à silagem de milho uma vez que não influenciou o desempenho animal ou o consumo de nutrientes. No entanto, a digestibilidade diminuiu com o aumento do nível de inclusão do subproduto na dieta.